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Prometheus NT.48 Nano Temper Technologies Protein Foling Analyser

Condition Used
Stock Number 45360 / 1500
This listing is for the Prometheus NT.48 Nano Temper Technologies Protein Foling Analyser

The unit is in full working condition and is ready for immediate release
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The Prometheus NT.48 is a cutting-edge, high-resolution instrument used for protein stability characterization. It utilizes a variety of label-free technologies to monitor both thermal and chemical stability, making it an invaluable tool in drug development, particularly for protein-based therapeutics like antibodies.


Here's a breakdown of its key features and functionalities:


Core Features:
Native DSF (Differential Scanning Fluorimetry): No dyes required for detection. It measures protein stability by detecting intrinsic fluorescence (tryptophan and tyrosine) during thermal or chemical denaturation.
Dual UV System: Uses two UV detectors to monitor fluorescence at 330 nm and 350 nm, providing high scanning speed and dense data points for ultra-high resolution measurements.
High-Resolution Detection: Capable of measuring subtle changes in unfolding transitions, even at very high concentrations or complex samples. This is crucial for detecting aggregation and monitoring thermal stability.
Sample Throughput: Can handle 48 samples simultaneously, making it suitable for high-throughput experiments such as buffer screening or multi-step profiling.
Broad Concentration Range: Works with samples ranging from 5 µg/mL to 250 mg/mL, making it suitable for a wide variety of applications, including early-phase antibody development and final formulation screening.


Temperature Range: From 15 °C to 95 °C, allowing for detailed temperature-dependent stability analysis
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Easy Handling: Requires only 10 µL of sample per condition, and features an intuitive software interface for easy operation and data analysis.
Maintenance-Free: Designed with capillary-based measurements for easy sample handling and durability, even under challenging sample conditions such as high viscosity formulations.
Advanced Features:
Backreflection Optics (Optional): Enhances the ability to detect aggregation in protein samples by measuring light scattering, particularly useful for studying protein aggregation in formulations.
High Sensitivity and Reproducibility: The capillary format and automatic internal referencing contribute to the instrument’s exceptional sensitivity and reproducibility, particularly for aggregation detection.
Wide Application: Can be used for both thermal and chemical denaturation and can analyze detergent-solubilized membrane proteins, highly concentrated formulations, and other complex biological samples.
Software and Compliance: Equipped with rmControl and bility Analysis software, supporting 21 CFR Part 11 compliance for use in regulated environments.
Applications:
Thermal Unfolding: Studies how proteins unfold as temperature increases, which can be crucial in drug design.
Chemical Unfolding: Measures stability in the presence of chemical denaturants, helping to identify compounds that destabilize proteins.
Aggregation Detection: Identifies the onset of aggregation, providing insights into protein formulation stability and quality control.
Protein Engineering and Formulation Screening: Ideal for optimizing protein formulations and screening for the best candidates based on stability data.